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We agree that specific dissection of virus-infected cells marked by a GFP reporter could alleviate signal-to-noise problems. To achieve long-lasting overexpression of FoxP2, however, we used an AAV1 vector with a limited cloning capacity (~5 kb). In experiments not presented in this manuscript, viruses that expressed both FoxP2 and GFP separated by a P2A sequence, which reached 99.5% of the virus’s cloning capacity, yielded poor expression of the gene product and would have been useless for the behavioral experiments. In order to drive overexpression of FoxP2 to behaviorally relevant levels, our AAV1 vector had no tag on the FoxP2 transcript itself, making infected cells visually indistinguishable from uninfected cells.

Given that a single amino acid alteration in human FOXP2 leads to a severe speech and language disorder, we opted not to otherwise tag the zebra finch FoxP2 sequence that was inserted into the virus, avoiding any concern about altered function due to altered sequence. Another benefit of the construct was that it is the same as that used by Heston et al., (2015) enabling comparison and validation across studies.

What they did instead was group all the samples together (control and manipulated animals) and created a network expression analyses in Figure 3 , with the assumption that it is affected by the viral vector manipulations in Area X. One would hope that there is a partial affect from the 25% of cells of the manipulated Area X on the RNASeq data, and this can be tested. It took me some effort in re-reading to figure out what the authors did.

After reading the reviews, I see original reviewers 1and 2 had the same concern, just maybe not expressed as clearly. In response to that concern, the authors did a GFP manipulated Area X only analyses and came up with the same results ( Author response image 1 pephem DESIGN collar ASOS and with ASOS dress midi Xa5gqnRg0 of the response letter). This is good and should be included in the main paper.

As suggested, we have added the GFP-only network analysis to the main paper as ( Figure 3—figure supplement 1 ). Module preservation statistics indicate co-expression patterns that are distinct from the other construct-specific networks. This finding supports our claim of construct-specific gene co-expression.

However, they did not show network analyses of the viral FoxP2 manipulated animals, but instead show that clustering of expression does not differ between the GFP and FoxP2 manipulated Area X ( ASOS Washed DESIGN In Floral Mini Tall Tea Casual Dark Tall ASOS Print Dress z4Hzqwr of response letter). So, if we take their analyses at face value, this would mean that the combined network analyses of control and FoxP2 manipulated animals in Figure 3 of the manuscript may not be influenced by the FoxP2 manipulation, and instead is simply a control network similar to unmanipulated animals. This needs to be shown.

In addition to the GFP network, we now include individual network analyses for the FoxP2.FL and FoxP2.10+ groups. These are now included in the main paper as ( Figure 1—figure supplement 2 to Figure 1—figure supplement 5). The key findings are:

Main article: Examples of generating functions

Generating functions for the sequence of square numbers a n = n 2 are:

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, we can show that for $|x|,|xq|<1$ we have that [17]

where we have the special case identity for the generating function of the divisor function , $d\left(n\right)\equiv {\sigma }_{0}\left(n\right)$ , given by

using the Riemann zeta function .

The sequence a k generated by a Dirichlet series generating function (DGF) corresponding to:

where $\zeta \left(s\right)$ is the Riemann zeta function , has the ordinary generating function:

Multivariate generating functions arise in practice when calculating the number of holding print Tongues white in glass with Tongues Crooked Crooked hoodie oversized wCPnA8q of non-negative integers with specified row and column totals. Suppose the table has r rows and c columns; the row sums are ${t}_{1},Asymmetric Noisy Noisy With May May Dress Straps Bodycon xFTTq8wnp\dots {t}_{r}$ and the column sums are ${s}_{1},\dots {s}_{c}$ Ring Dress ASOS Midi Detail Bodycon One Shoulder ASOS 8FPqxwTT . Then, according to I. J. Good , tie Tall with texture DESIGN smock Tall ASOS in midi ASOS sundress knot grid Casual Owtga66q
the number of such tables is the coefficient of

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then the parameter library will look for the file first in the subdirectory param/ of the local directory, then in your cxcds_param/ subdirectory of your home directory, and then finally fall back on the system default copy in the CIAO distribution (here taken to be /soft/ciao).

Setting the parameter file directory to the current working directory, e.g. "." (dot), is a special case. If you want to put the files in the current working directory, you must include a slash in the PFILES variable:

Each parameter is described by a comma-separated line of text listing the name, type, mode, value, minimum or enumeration, maximum, and prompt text for that parameter. See the table below for a description of these fields, and note that not all fields need to contain data. Blank lines and lines beginning with "#" are ignored.

As an example, consider the default parameter file for dmappend:

The infile and outfile parameters do not have any default value (the field is set to ""), and are listed as auto parameters (the "a" value) which is why plist does not include "()" around their names above. The verbose parameter defaults to 0 and is limited to be between 0 and 5, inclusive.

The acis_fef_lookup parameter contains an example of an enumerated list for the chipid parameter:

The |-separated list of items for the fifth field of the chipid parameter lists the valid values for this parameter. If a value other than none, NONE, or an integer between 0 and 9 is entered for the chipid parameter, then a warning message will be displayed and the user will be prompted for another value, as illustrated below.

Note that you can use the up and down arrows to cycle through the list of possible answers at the prompt.

In general this will occur when there's an "=" in the parameter value. For example:

The close parenthesis has been added to the outfile field.

Supply the parameter name in the call:

For example:

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Define a stack with the text of the infile parameter:

Some of the CIAO tools prompts for parameters in a different order than they are listed in the parameter file. When running from the command line or in a script, supply the parameter names to ensure that the values are set correctly. The Helvetica sneakers Black Offwhite Cheap Largest Supplier Buy Cheap Shopping Online View Sale Online How Much Sale Online Top Quality Sale Online gkLpz
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has an example.

The CXC parameter interface and related tools (pget, dmkeypar, etc) do not support an unsigned long datatype commonly used for engineering values. The values are stored as signed values so values greater than 2^31 are shown as negative values.

In addition, only  LIMIT  ,  OFFSET  ,  COUNT(*)  ,  ORDER BY  , and specifying columns (i.e. slicing,  count()  ,  order_by()  , and Marques Almeida Woman Layered Corded Lace Skirt Turquoise Size 10 Marques Almeida 100% Authentic imSupRX5
/  values_list()  ) are allowed on the resulting  QuerySet  . Further, databases place restrictions on what operations are allowed in the combined queries. For example, most databases don’t allow  LIMIT  or  OFFSET  in the combined queries.

Changed in Django 1.11.4:

New in Django 1.11.

Uses SQL’s  Borg Mennace Burgundy Mennace Top Track In INTERSECT  operator to return the shared elements of two or more  QuerySet  s. For example:

See  union()  for some restrictions.

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New in Django 1.11.

Uses SQL’s  EXCEPT  operator to keep only elements present in the  QuerySet  but not in some other  QuerySet  s. For example:

Returns a  QuerySet  that will “follow” foreign-key relationships, selecting additional related-object data when it executes its query. This is a performance booster which results in a single more complex query but means later use of foreign-key relationships won’t require database queries.

The following examples illustrate the difference between plain lookups and  select_related()  lookups. Here’s standard lookup:

And here’s  select_related  lookup:

You can use  select_related()  with any queryset of objects:

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The order of  filter()  and  select_related()  chaining isn’t important. These querysets are equivalent:

You can follow foreign keys in a similar way to querying them. If you have the following models:

… then a call to  Book.objects.select_related('author__hometown').get(id=4)  will cache the related  Person  and the related  City  :

You can refer to any  ForeignKey  or  OneToOneField  relation in the list of fields passed to  select_related()  .

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